Topic: whole mouse brain

3D ultrasound localization microscopy (3D-ULM) allows in vivo quantification of blood vessel velocities in the mouse brain using matrix arrays, but it requires complex electronics, and the field of view remains limited [1]. Here, we investigate how low channel count Row-Column Addressing (RCA) probes allow 3D-ULM to be performed in the whole mouse brain non-invasively and in the visual cortex of a non-human primate after craniotomy.An IcoPrime 4D-RCA probe (80+80 elements, 15 MHz, pitch 0.11 mm, Iconeus) was driven by an Iconeus One scanner. For the mouse experiment, the probe was mounted on a

Light-Sheet Fluorescence Microscopy (LSFM) is an excellent tool to study neuronal populations in large samples such as the whole mouse brain, thanks to its inherent three-dimensional nature and its high resolution down to the single cell. In this work we report on our latest technological developments in both hardware and software and their applications in several projects aiming at reconstructing the neuronal organization in both the human cortex and in whole mouse brain.Our latest LSFM setup is capable of simultaneous excitation and acquisition at four wavelengths, thus enabling fast reconst