Eukaryotic Genome Assembly using PacBio and Hi-C

Assemble eukaryotic genomes by combining long-read sequencing with chromatin-contact data. Practical work progresses from read manipulation and k-mer analysis through assembly algorithms, removal of duplicated haplotigs and Hi-C scaffolding. Participants assess contiguity, sequence accuracy and gene completeness with approaches including Merqury and BUSCO, and finish by comparing results from small eukaryotic datasets. The theoretical component explains how repeats, heterozygosity and sequencing technologies influence assembly choices.

Sessions: 9–13 November, 14:00–20:00 daily, Europe/Berlin (CET, UTC+01:00).

Live online training; fee €530. Suitable for researchers learning de novo assembly, including beginners; Linux practice is included. An optional €900 bundle includes the separate manual-curation course.