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A MULTIPROTEIN PROFILING STUDY ON NEUROIMMUNOLOGICAL SIGNATURES IN THE POST-MORTEM BRAIN OF DELIRIOUS PATIENTS
Aglaia Pozantziand 4 co-authors
Dept. Pathology and Medical Biology
FENS Forum 2026 (2026)
Barcelona, Spain
Board PS01-07AM-556
Presentation
Date TBA
Board: PS01-07AM-556
Event Information
Poster Board
PS01-07AM-556
Poster
View posterAbstract
Delirium is a frequent and severe neuropsychiatric syndrome that predominantly affects older hospitalized patients and is associated with increased risk for comorbidities, dementia, and mortality. While clinicians recognize its clinical importance, the underlying molecular and neuroimmune alterations associated with delirium remain unknown in the brain tissue. We aimed to identify neuroimmune markers that are associated with delirium onset, in human brain autopsy tissues.
Formalin-fixed paraffin-embedded brain sections from 14 patients hospitalized due to severe inflammation (8 delirious cases, 6 non-delirious controls) were stained using immunohistochemistry for a multiprotein panel reflecting neuroimmune activation, regulation, and neurodegenerative processes (Iba-1, GFAP, HLA-DR, PD-1, PD-L1, VISTA, CD3, CD4). Using digital image analysis, we quantified staining intensity, expressed as Pixel Density Score (PDS) of PD1 and compared it across hippocampal regions and the thalamus between cases and controls.
In both cases and controls, PD-1 was expressed by pyramidal and granular neurons, endothelial and smooth muscle cells, macrophages and granulocytes. Delirium patients exhibited significantly higher PD-1 PDS in the CA4 hippocampal region (p=0.026) and a trend for higher PDS appeared in the other hippocampal subfields. Stainings against Iba-1,GFAP, HLA-DR, PDL-1, VISTA, CD3 and CD4 showed positive signal in all tissues and quantification analyses are ongoing.
The increased PD-1 PDS in delirious cases indicates altered immunological regulation in the brains of delirious patients. Quantifying the remaining proteins of interest will help to reveal a delirium-associated proteomic pattern.
Formalin-fixed paraffin-embedded brain sections from 14 patients hospitalized due to severe inflammation (8 delirious cases, 6 non-delirious controls) were stained using immunohistochemistry for a multiprotein panel reflecting neuroimmune activation, regulation, and neurodegenerative processes (Iba-1, GFAP, HLA-DR, PD-1, PD-L1, VISTA, CD3, CD4). Using digital image analysis, we quantified staining intensity, expressed as Pixel Density Score (PDS) of PD1 and compared it across hippocampal regions and the thalamus between cases and controls.
In both cases and controls, PD-1 was expressed by pyramidal and granular neurons, endothelial and smooth muscle cells, macrophages and granulocytes. Delirium patients exhibited significantly higher PD-1 PDS in the CA4 hippocampal region (p=0.026) and a trend for higher PDS appeared in the other hippocampal subfields. Stainings against Iba-1,GFAP, HLA-DR, PDL-1, VISTA, CD3 and CD4 showed positive signal in all tissues and quantification analyses are ongoing.
The increased PD-1 PDS in delirious cases indicates altered immunological regulation in the brains of delirious patients. Quantifying the remaining proteins of interest will help to reveal a delirium-associated proteomic pattern.
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