Analysing RNA structure-probing data with the rnastructurome nf-core pipeline

Chemical probing experiments such as SHAPE and DMS reveal RNA structure by measuring how individual nucleotides react with a probe. This webinar introduces nf-core/rnastructurome, a Nextflow pipeline developed by the RNAcentral team with partners in the RNA structure community, which supports reverse-transcription-stop and mutational-profiling experiments. Starting from reads and a sample sheet, it performs quality control and alignment, computes normalised per-nucleotide reactivities and uses them to guide secondary-structure prediction; demonstrations cover inputs, QC reports, reactivity tracks, structure diagrams and how controls and replicates help assess results.