DNA methylation

…It covers DNA methylation, histone and RNA modifications, non-coding RNAs, heterochromatin, nucleosome structure and nuclear architecture. Researchers will connect transcriptional control with developmental processes and disease mechanisms, using single-cell genomics and advanced microscopy alongside molecular approaches. The programme also considers how ageing, environmental exposures and lifestyle influence epigenetic states. The meeting brings together researchers working across

SYNGAP1-related encephalopathy is a rare neurodevelopmental disease characterized by diverse genetic mutations and phenotypic features. It is not known whether the blood-brain barrier (BBB) is affected in SYNGAP1 patients, therefore, this study aimed to establish a human induced pluripotent stem cell (hiPSC)-based BBB model of the SYNGAP1 disorder and identify relevant functional and molecular signatures. To establish the BBB models, SYNGAP1 knockout (KO), patient-derived hiPSC lines (Leu323Arg) and isogenic controls were differentiated into brain capillary endothelial-like cells and seeded on

…DNA methylation can act as a long-term regulatory signal, therefore being a prime candidate to regulate memory duration and stabilisation within engrams– the physical substrate of a memory.Using contextual fear conditioning (CFC) and engram tagging tools in mice, we showed that reactivation of cortical engrams reflects sys

The crisis in youth mental health has intensified, especially after the COVID-19 pandemic. Traditional assessment tools like the Highly Sensitive Person – HSP index (1) and the Perceived Stress Scale – PSS (2) provide valuable insights but may overlook the multifaceted nature of mental health. Thus, incorporating molecular biomarkers becomes crucial to unravel the interplay of genetic, environmental, and psychological factors. In this work, we combined neuropsychological and epigenetic data in a machine learning algorithm to predict HSP scores in university students and to provide individualis

…in schizophrenia, and have genomic methylation changes enriched for oligodendrocyte genes.Aims: Examine the relationship between mIA and altered oligodendrocyte development and how these correspond to the onset of adult

…(THC) on epigenetic DNA modifiers conducting DNA methylation and demethylation (DNMTs and TET enzymes) on human peripheral mononuclear cells (PBMCs) in vitro. Additionally, we profiled the genome-wide effect of THC and assessed if any genes with marked changes in methylation or hydroxymethylation could be identified. Changes in DNA methylatio

…key for methyl group generation for methylation of DNA and histones. Our study aimed to understand how ELS and early micronutrient intervention affect (i) HPA-axis responsivity and anxiety/depressive-like behavior, and (ii) hypo

…In addition, DNA methylation was shown in adults to be an important epigenetic mechanism involved in HD. Here we sought to explore early pre-symptomatic neurodevelopmental alterations in DNA methylation using cerebral organoids from juvenile forms of isogenic HD pluripotent cells. We find wide-spread DNA demethylation in the mutant ce

Distinct patterns of DNA methylation (mC) and hydroxymethylation (hmC) are identified in the neuronal epigenome, and the dynamic regulation of mC/hmC is associated with various hippocampus-dependent memory processes. hmC, an oxidized form of mC, acts as an essential intermediate in the active DNA demethylation pathway. The further oxidized hmC derivatives are eventually removed by the thymine DNA glycosylase (TDG) initiated base excision repair (BER). Constitutive knockout or catalytic inactivation of TDG leads to embryonic lethality in mice, demonstrating an essential role of TDG in the epige

…DNA methylation levels of serotonin transporter (SLC6A4m) and tryptophan hydroxylase 2 (TPH2m) genes have been suggested as predictive biomarkers of antidepressant efficacy. In this study, we aimed to evaluate SLC6A4m/TPH2m biomarker potential and compare with previous findings in an independent cohort of N=77 patients with major depressive disorder who were treat

…regions (ICR) that are differentially DNA methylated regions (DMRs) on the two

…pronounced alterations of neuronal DNA methylation patterns in the hippoca

…changes in histone modifications, DNA methylation, and hydroxymethylation, accompanied by transcriptomic profiling in cell and animal models of PD as well as large patient cohorts. Furthermore, we assess the plasticity of epigenomic modifications under influence of envi

…mapping of enhancer activity, DNA methylation and the 3D genome landscape in vivo, we dissected how the epigenome is rewired during cortical development. We identified and functionally validated key transcription factors such as Neurog2 which underlie regulatory dynamics and coordinate rewiring across multiple epigenetic layers to ensure ro

The inhibitory actions of the heterogeneous collection of GABAergic interneurons tremendously influence cortical information processing, which is reflected by diseases like autism, epilepsy and schizophrenia that involve defects in cortical inhibition. Apart from the regulation of physiological processes like synaptic transmission, proper interneuron function also relies on their correct development. Hence, decrypting regulatory networks that direct proper cortical interneuron development as well as adult functionality is of great interest, as this helps to identify critical events implicated