Topic: ferroptosis

Microglial phenotype during Alzheimer’s Disease (AD) continuum is under debate. At early stages, a protective phenotype with the typical DAM profile would participate in plaque compaction and Aβ degradation. Instead, human AD microglia (HAM) show an enhance aged/degenerative profile, supporting a microglial dysfunction as a central mechanism in AD aetiology. Recent findings from our lab described that peri-plaque microglia are highly reliant on oxygen levels and mitochondrial integrity. Nevertheless, mitochondrial implication in aged microglia during the disease progression remains unclear. Th

Parkinson’s disease (PD) is the second most common neurodegenerative disorder characterized by the degeneration of dopaminergic neurons of the Substantia Nigra and the accumulation of protein aggregates, called Lewy bodies, where the most abundant protein is alpha-synuclein (a-SYN). In addition to the neurodegeneration and the accumulation of proteins, PD is characterized by chronic low-grade inflammation and mitochondrial alterations that lead to oxidative stress. Currently, the only treatment for PD patients is dopamine replacement therapy (levodopa), which has serious side effects and does

Intracerebral haemorrhage (ICH) is the second-most common form of stroke. Due to the post-ICH brain damage and limited therapeutic strategies, the disability rate among ICH survivors is high. DJ-1 is a protein known to play roles in cytoprotection. However, DJ-1 can be oxidised and lose its protective functions. Our study investigated the oxidation state of DJ-1 after ICH and the therapeutic potential of DJ-1 oxidation inhibitor, DJ-1-binding compound 23 (Comp-23), in ICH. We induced experimental ICH mice models by intra-striatal injection of collagenase. Immunoblot, qPCR measurement and immun

Aims: Excessive oxidative stress is a major factor to damage retinal pigment epithelial (RPE) cells that promotes the development of retinal degeneration. We investigated the effects of 6dS, a pigment epithelium-derived factor (PEDF) derived factor on cultured RPE cells and retina of rats treated with sodium iodate (NaIO3). Methods: Annexin V/PI staining was used to determine ARPE-19 cell viability. FerroOrange and immunostaining of acrolein were used to detect intracellular labile ion (Fe2+)-dependent cell ferroptosis and lipid peroxidation reaction in plasma membrane. Immunoblotting and real