Topic: Neuropixels

The Stagkourakis Lab at Karolinska Institutet is recruiting a postdoctoral scholar to study neural circuits governing survival and homeostatic behaviors. The project combines systems neuroscience, Neuropixels recordings, imaging, circuit manipulation and transcriptomics in the SciLifeLab and Department of Neuroscience research environment.

The Allen Institute for Neural Dynamics seeks a Research Associate I to record thousands of neurons across brain regions while mice perform cognitive and foraging tasks. The role uses Neuropixels electrophysiology, optogenetics, optical physiology, rodent behavior, histology, and data-quality analysis within a collaborative brain-wide-circuit programme.

The cortex comprises many neuronal types, which can be distinguished by their transcriptomes: the sets of genes they express. Little is known about the in vivo activity of these cell types, particularly as regards the structure of their spike trains, which might provide clues to cortical circuit function. To address this question, we used Neuropixels electrodes to record layer 5 excitatory populations in mouse V1, then transcriptomically identified the recorded cell types. To do so, we performed a subsequent recording of the same cells using 2-photon (2p) calcium imaging, identifying neurons b

Most brain functions involve interactions among multiple, distinct areas or nuclei. Yet our understanding of how populations of neurons in interconnected brain areas communicate is in its infancy. Using a population approach, we found that interactions between early visual cortical areas (V1 and V2) occur through a low-dimensional bottleneck, termed a communication subspace. In this talk, I will focus on the statistical methods we have developed for studying interactions between brain areas. First, I will describe Delayed Latents Across Groups (DLAG), designed to disentangle concurrent, bi-

Neural decoding is essential for understanding the association between neural activity and behavior. A prerequisite for most decoding methods is spike sorting, the assignment of action potentials (or spikes) to individual neurons. Current spike sorting algorithms, however, can be inaccurate and do not properly model uncertainty of spike assignments, therefore discarding information that could potentially improve decoding performance. Recent advances in high-channel-count probes like Neuropixels (NP) and extracellular analysis pipelines allow for extracting a rich set of spike features to direc

During navigation, animals estimate their position using path integration and landmarks. In a series of two studies, we used virtual reality and electrophysiology to dissect how these inputs combine to generate the brain’s spatial representations. In the first study (Campbell et al., 2018), we focused on the medial entorhinal cortex (MEC) and its set of navigationally-relevant cell types, including grid cells, border cells, and speed cells. We discovered that attractor dynamics could explain an array of initially puzzling MEC responses to virtual reality manipulations. This theoretical framewo

How psychedelic drugs change the activity of cortical neuronal populations is not well understood. It is also not clear which changes are specific to transition into the psychedelic brain state and which are shared with other brain state transitions. Here, we used Neuropixels probes to record from large populations of neurons in prefrontal cortex of mice given the psychedelic drug TCB-2. The primary effect of drug ingestion was stretching of the distribution of log firing rates of the recorded population. This phenomenon was previously observed across transitions between sleep and wakefulness,

Mammalian vision and visually-guided behavior relies on neurons distributed across diverse brain regions. In this talk I will describe our efforts to create tools that allow us to measure activity from these distributed circuits - Neuropixels probes for large-scale electrophysiology - and our findings from studies deploying these tools to study visual detection and discrimination in mice.

Join us for a comprehensive introduction to multielectrode recording technologies for in vivo neurophysiology. Whether you are new to the field or have experience with one type of technology, this webinar will provide you with information about a variety of technologies, with a main focus on Neuropixels probes. Dr Kris Schoepfer, US Product Specialist at Scientifica, will provide an overview of multielectrode technologies available to record from one or more brain areas simultaneously, including: DIY multielectrode probes; Tetrodes / Hyperdrives; Silicon probes; Neuropixels. Dr Sylvia Schröd

Psychedelics (5-HT2AR agonists) show great promise in treating mental disorders but their effects on neuronal population activity are not clear. Latent dimensions identified by Principal Component Analysis (PCA) capture the dynamics of this interrelated neuronal population activity. We asked whether latent dimensions emerge or disappear as a result of psychedelic administration (acute), and across other naturally occurring brain state transitions (sleep, arousal). Contrastive PCA (cPCA) [Abid et al. (2018), Nature Comm.] was applied to spontaneous brain activity recordings in rodents before