Transcription factors

…how mechanical environments activate transcription factors, move regulatory molecules into nuclei, couple the cytoskeleton to nuclear architecture, alter chromatin dynamics and influence genome maintenance and disease. High-resolution imaging, live-cell tracking, engineered microenvironments, ultrasensitive detection, single-cell and spatial omics, and computational models provide complementary approaches. Physicists, engineers, computer scientists, biologists and clinicians wil

Different brain cell types exhibit distinct metabolic signatures that link energy economy to cellular function. Astrocytes and neurons, for instance, diverge dramatically in their reliance on glycolysis versus oxidative phosphorylation, underscoring that metabolic fuel efficiency is not uniform across cell types. A key factor shaping this divergence is the structural organization of the mitochondrial respiratory chain into supercomplexes. Specifically, complexes I (CI) and III (CIII) form a CI–CIII supercomplex, but the degree of this assembly varies by cell type. In neurons, CI is predominant

This lecture examines the sequence features through which enhancers bind transcription factors and control gene expression. It introduces computational approaches, including neural networks, for recognising regulatory DNA and potentially designing synthetic elements. It also compares recurring arrangements of enhancers around genes important in mammalian development.

…disease (PD), may interact with transcription factors and affect gene transcription. Researchers have revealed that Aβ1-42 can be found in the nucleus, interact with DNA, and change the expression of many genes. It is known that α-synuclein functions as a transcription factor that binds to nuclear DNA and modulates the expression of many genes. Our study aims to investigate whether these peptides directly affect the expression of

…Methods: To investigate the factors involved in metabolic switch, we used transcriptomics analysis on the sensory neurons cultured in different metabolic demand. IPA analysis was used to interpret the transcriptomics data. We applied MEME-ChIP method on comprehensive motif an

…we aim to investigate the role of transcription factors in specifying neural fate and regulating neural lineage progression in the developing thalamus. Using a co

…lineages, as well as antibodies to transcription factors and functional proteins of nervous system were studied on the material from the Collection of prenatal human development of AP. Avtsyn Research Institute of Human Morphology, Moscow. In 2021, the online project dedicated to the Collection had been granted by the FENS History Committee(https://brai

The extracellular matrix protein reelin, named after the reeler-mutant mouse, exerts a multitude of key functions in both the developing and adult brains of mammals. In addition to the well-studied effect of reelin on the migration of neuronal cells during embryonic development, several studies have demonstrated an effect of reelin on neuronal signaling in the mature brain. By modulating both excitatory glutamatergic and inhibitory GABAergic neuronal signaling, reelin affects central neurophysiological processes such as learning and memory formation. We aim to further investigate reelin-induce

Charting the regulatory interactions between transcription factors (TFs), enhancers, and genes during the formation of neuronal lineages in human can help decipher the complex genetic etiologies of neurodevelopmental disorders such as autism spectrum disorder (ASD). To achieve this, we conducted an integrative analysis of epigenomic and transcriptomic profiles of human forebrain organoids derived from individuals with idiopathic ASD and their neurotypical fathers. This multi-omics profiling allowed us to build an enhancer-driven gene regulatory network of early neural development and relate it

One of the most prominent features of the human brain is the fabulous size of the cerebral cortex and its intricate folding, both of which emerge during development. Over the last few years, work from my lab has shown that specific cellular and genetic mechanisms play central roles in cortex folding, particularly linked to neural stem and progenitor cells. Key mechanisms include high rates of neurogenesis, high abundance of basal Radial Glia Cells (bRGCs), and neuron migration, all of which are intertwined during development. We have also shown that primary cortical folds follow highly stereot

…if they force-express neurogenic transcription factors or miRNAs. Direct astrocytic reprogramming to induced-neurons (iNs) is a powerful approach for manipulating cell fate, as it takes advantage of the intrinsic neural stem cell (NSC) potential of brain resident reactive astr

…alters the localization of two transcription factors, likely releasing an insulin-like peptide from the intestine, which in turn modifies chemosensory neurons and alters behavior. These results present a model for how inter-tissue signals to generate flexible behaviors via gut-brain signaling.

…and functionally validated key transcription factors such as Neurog2 which underlie regulatory dynamics and coordinate rewiring across multiple epigenetic layers to ensure ro