Histology

Four-year doctoral position in the Ultra-High Field Neuroimaging group and Spinoza Centre for Neuroimaging. The project maps human cerebellar structure in postmortem brain tissue to provide a reference for in-vivo ultra-high-field MRI experiments. Research combines tissue staining, microscopy, quantitative image analysis and postmortem MRI, with access to 7T imaging and the Netherlands Brain Bank. The advertised appointment is full-time in Amsterdam.

Brain metabolism supplies the energy underlying brain injury, recovery and aging. This webinar brings together three complementary approaches to understanding how repetitive mild traumatic brain injury affects these processes. Sakthivel Ravi examines repetitive mild TBI and aging through multimodal imaging and molecular profiling. Adam Khalifa presents wireless brain glucose sensing for TBI research, and Joe Abisambra discusses disruption of bioenergetic pathways following repetitive mild TBI. The talks are followed by a moderated question-and-answer discussion.

Develop antibody-based approaches to Alzheimer’s disease at the Queensland Brain Institute. The project investigates tau-targeting monoclonal antibodies through protein production, cell-based experiments and mouse studies. Responsibilities include analysing results, preparing publications, contributing to funding applications and supporting junior researchers. This full-time appointment runs for up to 12 months, with an immediate start preferred. Annual base pay is AUD 85,373–113,660, plus 17% superannuation.

Matthew J. Girgenti discusses how research on individual brain cells and the three-dimensional organization of DNA is changing the biological understanding of post-traumatic stress disorder. The seminar examines how these approaches reveal trauma-associated brain changes and identify possible targets for more effective, personalized treatments. Girgenti is a neuroscientist in Yale School of Medicine’s Department of Psychiatry. Jeffrey Borenstein moderates this Meet the Scientist session. Online via the BBRF Zoom webinar. Tuesday 13 October 2026, 14:00 EDT (America/New_York; UTC−4). Follow Register Today on the organizer’s event page to the event-specific Zoom form. Advance registration is available to researchers, students, healthcare providers, mental-health organizations, patients and family members.

The standard method for staining structures in the brain is to slice the brain into 2D sections. Each slice is treated using a technique such as in-situ hybridization to examine the spatial expression of a particular molecule at a given developmental timepoint. Depending on the brain structures being studied, slices can be made coronally, sagitally, or at any angle that is thought to be optimal for analysis. However, assimilating the information presented in the 2D slice images to gain quantitiative and informative 3D expression patterns is challenging. Even if expression levels are presented as voxels, to give 3D expression clouds, it can be difficult to compare expression across individuals and analysing such data requires significant expertise and imagination. In this talk, I will describe a new approach to examining histology slices, in which the user defines the brain structure of interest by drawing curves around it on each slice in a set and the depth of tissue from which to sample expression. The sampled 'curves' are then assembled into a 3D surface, which can then be transformed onto a common reference frame for comparative analysis. I will show how other neuroscientists can obtain and use the tool, which is called Stalefish, to analyse their own image data with no (or minimal) changes to their slice preparation workflow.

Wasteosomes (or corpora amylacea) appear in the human brain with advancing age and in some neurodegenerative diseases. They have been proposed to accumulate waste substances and to contribute to a brain-cleaning mechanism. In this context, wasteosomes may have the potential to entrap some of the proteins that misfold, aggregate and accumulate in neurodegenerative diseases. In this work, we aim to characterize wasteosomes in the three primary frontotemporal lobar degeneration (FTLD) proteinopathies: FTLD with tau (FTLD-tau), FTLD with TAR DNA-binding protein 43 (FTLD-TDP) and FTLD with Fused in Sarcoma (FTLD-FUS). Cryopreserved human hippocampal sections were obtained from 4 cases of FTLD-tau, 3 cases of FTLD-TDP, and 3 cases of FTLD-FUS. Immunofluorescence methods with prior antigen retrieval were applied to detect tau, phosphorylated-TDP-43 (pTDP-43) and FUS in wasteosomes. Our results revealed the presence of tau, pTDP-43 and FUS in wasteosomes from FTLD-tau, FTLD-TDP, and FTLD-FUS, respectively. The staining of tau and pTDP-43 was placed in the peripheral part of the wasteosomes, while that of FUS was located in their central part. These results corroborate that wasteosomes may entrap misfolded proteins and indicate that the composition of wasteosomes differs depending on the proteinopathy. We conclude that wasteosomes in FTLD can contain tau, TDP-43 or FUS in concordance with the affected protein. Moreover, since there is currently no established biomarker for the diagnosis of FTLD, we propose to study the presence of these proteins in wasteosomes obtained from the cerebrospinal fluid as a potential tool diagnostic for FTLD.

Africa is blessed with a rich diversity and abundance in rodent and avian populations. This natural endowment on the continent portends research opportunities to study unique anatomical profiles and investigate animal models that may confer better neural architecture to study neurodegenerative diseases, adult neurogenesis, stroke and stem cell therapies. To this end, African researchers are beginning to pay closer attention to some of her indigenous rodents and birds in an attempt to develop spontaneous laboratory models for homegrown neuroscience-based research. For this presentation, I will be showing studies in our lab, involving cellular neuroanatomy of two rodents, the African giant rat (AGR) and Greater cane rat (GCR), Eidolon Bats (EB) and also the Striped Owl (SO). Using histological stains (Cresyl violet and Rapid Golgi) and immunohistochemical biomarkers (GFAP, NeuN, CNPase, Iba-1, Collagen 2, Doublecortin, Ki67, Calbindin, etc), and Electron Microscopy, morphology and functional organizations of neuronal and glial populations of the AGR , GCR, EB and SO brains have been described, with our work ongoing. In addition, the developmental profiles of the prenatal GCR brains have been chronicled across its entire gestational period. Brains of embryos/foetuses were harvested for gross morphological descriptions and then processed using immunofluorescence biomarkers to determine the pattern, onset, duration and peak of neurogenesis (Pax6, Tbr1, Tbr2, NF, HuCD, MAP2) and the onset and peak of glial cell expressions and myelination in the prenatal GCR. The outcome of these research efforts has shown unique neuroanatomical expressions and networks amongst Africa’s rich biodiversity. It is hopeful that continuous effort in this regard will provide sufficient basic research data on neural developments and cellular neuroanatomy with subsequent translational consequences.

Lead experimental studies that identify and validate targets for neurodegenerative disease. Design rodent experiments, analyse human and animal brain tissue, and develop quantitative measures of protein aggregation, inflammation, cell loss and vascular integrity. Collaborate with pharmacology, histology, molecular biology and computational teams to turn mechanistic findings into programme decisions. The employer’s current vacancy accepts applications without a stated fixed deadline.

E11 Bio is hiring a hands-on senior research associate to own rodent perfusion, fixation, stereotaxic and retro-orbital injection, tissue-quality optimization, and animal-colony workflows. The position supports an interdisciplinary neuroscience program developing scalable mammalian brain mapping and connectomics technology.

The Allen Institute's Brain Health team seeks a Research Associate II to characterize AAV vectors that target specific brain-cell types across mouse, non-human-primate, and human tissue. Work includes brain-tissue processing, RNAscope and immunohistochemistry, fluorescence microscopy, image analysis, and support for robotic in-vivo viral-injection studies.